Lumiprobe
2024-10-23自2006年以來,我們生產(chǎn)和銷售用于生命科學(xué)研究和診斷的高級(jí)化學(xué)品。我們的目錄包括熒光染料、用于寡核苷酸合成的亞磷酰胺、點(diǎn)擊化學(xué)品和其他試劑。大多數(shù)物品都有庫存,隨時(shí)可以運(yùn)往世界各地。
NMR. Nuclear magnetic resonance is the basic method for the identification of organic compounds. We routinely use 1H NMR for identity and purity checking. For phosphoramidites and triphosphates, 31P NMR is used, too. And we also measure other nuclei (13C, 19F, etc) for all compounds which we prepare for the first time to identify them unambiguously. Two-dimensional NMR (such as HSQC and HMBC) are used for complicated cases. For example, we attributed rhodamine isomers with 2D NMR - something impossible to do with one-dimensional NMR spectra.
HPLC-MS. High pressure liquid chromatography coupled with mass spectrometry is a powerful method which we also routinely use for identity and purity checking. It gives quantitative measure of compound purity along with the measurement of its molecular weight.
This system that we have in house provides both UV spectra, and mass spectra for all of the components of the analyzed mixture - so we check online absorbance spectra of dyes, as well as their molecular weights.