流式補(bǔ)償微球-用于多色流式細(xì)胞儀分析的熒光補(bǔ)償設(shè)置
2025-04-19SPHERO? COMPtrol: Fluorescent Conjugate QC and Antibody Binding Compensation Particles
- Coated with Goat anti-Mouse Ig to bind to the fluorochrome-conjugated monoclonal antibodies used for cell staining
- Provide a method for the quality control of the fluorochrome-conjugated monoclonal antibodies
- Aid in setting proper compensation to reduce the cross-talk between the channels of multicolor flow cytometers.
| 貨號(hào) | 品名 | 濃度 | 粒徑 | 規(guī)格 | 備注 |
| SPBY-CMIgP-08-2K | COMPtrol Kit, Goat anti-Mouse Ig (H&L) Coated Particles,2 populations (Negative & High) | 1.0x107/mL | 0.7-0.9 μm | 2x5 mL | 相當(dāng)于invitrogen的OneComp eBeads? |
| SPBY-CMIgP-30-2K | COMPtrol Kit, Goat anti-Mouse Ig (H&L) Coated Particles,2 populations (Negative & High in separate viales) | 1.0x107/mL | 3.0-3.4 μm | 2x5 mL | 相當(dāng)于invitrogen的OneComp eBeads? |
| SPBY-CMIgP-30-5 | COMPtrol Kit, Goat anti-Mouse Ig (H&L) Coated Particles,2 populations (Negative & High) | 1.0x107/mL | 3.0-3.4 μm | 5 mL | 相當(dāng)于invitrogen的OneComp eBeads? |
| SPBY-CMIgP-50-3K | COMPtrol Kit, Goat anti-Mouse Ig (H&L) Coated Particles,3 populations (Negative, Low, & High) | 2.5x106/mL | 5.0-5.9 μm | 3x5 mL | 相當(dāng)于invitrogen的OneComp eBeads? |
| SPBY-CMIgP-70-3K | COMPtrol Kit, Goat anti-Mouse Ig (H&L) Coated Particles,3 populations (Negative, Low, & High) | 2.5x106/mL | 7.0-7.9 μm | 3x5 mL | 相當(dāng)于invitrogen的OneComp eBeads? |
SPHERO? EasyComp: Spectral Matching Particles
- Surface labeled polystyrene particles with commonly used fluorophores
- Used for setting compensation in a wide variety of channels for any flow cytometer
- Supplied as individual products or as kits for use in different models of flow cytometers
| 貨號(hào) | 品名 | 濃度 | 粒徑 | 規(guī)格 | 備注 |
| SPBY-ECFP-B | EasyComp Fluorescent Particles, Blank | 107/mL | 3.0-3.4 μm | 5 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-F1 | EasyComp Fluorescent Particles, FITC | 107/mL | 3.0-3.4 μm | 1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-F2 | EasyComp Fluorescent Particles, PE | 107/mL | 3.0-3.4 μm | 1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-F3 | EasyComp Fluorescent Particles, PE-TR | 107/mL | 3.0-3.4 μm | 1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-F4 | EasyComp Fluorescent Particles, PE-Cy5 | 107/mL | 3.0-3.4 μm | 1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-F5 | EasyComp Fluorescent Particles, PE-Cy7 | 107/mL | 3.0-3.4 μm | 1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-F6 | EasyComp Fluorescent Particles, APC | 107/mL | 3.0-3.4 μm | 1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-F7 | EasyComp Fluorescent Particles, APC-Cy7 | 107/mL | 3.0-3.4 μm | 1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-F8 | EasyComp Fluorescent Particles, PerCP | 107/mL | 3.0-3.4 μm | 1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-F9 | EasyComp Fluorescent Particles, GFP | 107/mL | 3.0-3.4 μm | 1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-K1 | EasyComp Fluorescent Particle Kit (Blank, FITC, PE & PE-Cy5), 4 vials | 107/mL | 3.0-3.4 μm | 1x5 mL,3x1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-K2 | EasyComp Fluorescent Particle Kit (Blank, FITC, PE, PE-Cy5 & APC), 5 vials | 107/mL | 3.0-3.4 μm | 1x5 mL,4x1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-K3 | EasyComp Fluorescent Particle Kit (Blank, FITC, PE, PE-TR & PE-Cy5), 5 vials | 107/mL | 3.0-3.4 μm | 1x5 mL,4x1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
| SPBY-ECFP-K4 | EasyComp Fluorescent Particle Kit (Blank, FITC, PE, PE-TR, PE-Cy5, PE-Cy7, APC & APC-Cy7), 8 vials |
107/mL | 3.0-3.4 μm | 1x5 mL,7x1 mL | 熒光微球做補(bǔ)償，無(wú)需抗體，操作簡(jiǎn)單 |
Flow Cytometry Antibody Binding Beads
Single population Protein A or Protein G microspheres are suitable for labeling with conjugated antibodies from a range of hosts. When bound to your fluorecently labeled antibody, these microspheres may be used as single-population for removing spectral overlap of fluorochromes.
| 貨號(hào) | 品名 | 濃度 | 粒徑 | 規(guī)格 | 備注 |
| 553 | Flow Cytometry Protein A Antibody Binding Beads | 2.0e+6 beads/mL | 7-9μm | 1 mL | 可與大多數(shù)熒光一抗結(jié)合 |
| 554 | Flow Cytometry Protein G Antibody Binding Beads | 2.0e+6 beads/mL | 7-9μm | 1 mL | 可與大多數(shù)熒光一抗結(jié)合 |
Simply Cellular? Compensation Standards
Polystyrene beads coated with secondary antibodies that allow you to attach your fluorophore-conjugated mouse, rat, or human antibody. Once bound, these microspheres may be used as single-population for adjusting flow cytometers to avoid spectral overlap.
| 貨號(hào) | 品名 | 濃度 | 粒徑 | 規(guī)格 | 備注 |
| 550 | Simply Cellular? Compensation Standard (anti-Mouse IgG) | 2.0e+6 beads/mL | 7-9μm | 1 mL | 相當(dāng)于invitrogen的OneComp eBeads? |
| 556 | Simply Cellular? Compensation Standard - High (anti-Mouse IgG) | 2.0e+6 beads/mL | 7-9μm | 1 mL | 相當(dāng)于invitrogen的OneComp eBeads? |
| 551 | Simply Cellular? Compensation Standard (anti-Rat IgG) | 2.0e+6 beads/mL | 7-9μm | 1 mL | 相當(dāng)于invitrogen的OneComp eBeads? |
| 552 | Simply Cellular? Compensation Standard (anti-Human IgG) | 2.0e+6 beads/mL | 7-9μm | 1 mL | 相當(dāng)于invitrogen的OneComp eBeads? |
| 835 | Simply Cellular? anti-Mouse for Violet Laser (anti-Mouse IgG) | 2.0e+6 beads/mL | 5μm | 1 mL | 相當(dāng)于invitrogen的OneComp eBeads? |
Viability Dye Compensation Standard
Viability Dye Compensation Standards are suitable for labeling with LIVE / DEAD? stains or other amine-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX?, and users should contact us for discussion if uncertain as to the compatibility of a specific dye or stain. Supplied at ~1×107 beads/mL.
| 貨號(hào) | 品名 | 濃度 | 粒徑 | 規(guī)格 | 備注 |
| 450 | Viability Dye Compensation Standard, 4μm | 1 e+7 beads/mL | 4μm | 1 mL | LIVE/DEAD?補(bǔ)償用 |
| 451 | Viability Dye Compensation Standard, 8μm | 1 e+7 beads/mL | 8μm | 1 mL | LIVE/DEAD?補(bǔ)償用 |
FITC/PE Compensation Standard
The FITC / PE Compensation Standard includes four microsphere populations that are ~7-9 μm in diameter, including an Autofluor? population, and single populations of surface-labeled beads with FITC, PE, and FITC / PE. The Autofluor population are unlabeled beads exhibit autofluorescence at approximately the same intensity as unlabeled lymphocytes.
| 貨號(hào) | 品名 | 濃度 | 粒徑 | 規(guī)格 | 備注 |
| 820 | FITC/PE Compensation Standard (Autofluor?, FITC, PE, FITC/PE) | 2.0e+6 beads/mL | 7-9μm | 1 mL | 專用于FITC 和PE熒光補(bǔ)償用 |