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產品介紹
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背景描述：Insulin like Growth Factors 1 (IGF-I) is a 7.79 kDa member of the Insulin-like Growth Factors with 71 amino acid residues. IGF-I is mainly expressed from liver, adipose tissue, Endometrial stromal cells, Leydig cells, and can be isolated from plasma. IGF-I mediating the protein anabolic and promoting effect of pituitary growth hormone. IGF-I also affects metabolism of glycogen, DNA synthesis and glucose uptake via binding to IGF-I receptor.
別稱：insulin-like growth factor-I, Somatamedin C, IGF-IA
蛋白編碼 ：P05019
分子量：The protein has a calculated MW of 8.59 kDa. The protein migrates as 8-10 kDa under reducing condition (SDS-PAGE analysis).
表達系統：Escherichia coli
純度：>95% as determined by SDS-PAGE and SEC-HPLC.
生物活性：Measure by its ability to induce MCF-7 cells proliferation. The ED?? for this effect is 0.9-3.1 ng/mL. The specific activity of recombinant human IGF-I is approximately >1.2 x 103 IU/mg.
內毒素檢測：<0.01 EU per 1 μg of the protein by the LAL method.
蛋白序列：MGPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA with polyhistidine tag at the C-terminus.
蛋白標簽：His Tag (C-term)
產品形式：The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
產品應用：Cell Culture
產品使用：Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H?O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
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